Maintain relatively sterile technique without expensive lab equipment.
Liquid culture syringes contain mushroom mycelium suspended in a nutrient broth. After sterilizing the needle with a flame, you can transfer your culture to more nutrient broth, agar, sterilized grains, or bulk substrates. You can transfer with great success in the open air of your house. To further increase sterility, transfer within a glove box or in front of a flow hood.