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This mix of light malt extract with agar is a great nutrient base for culturing fungal mycelium in a petri dish. Ingredients: 50% Light Malt Extract, 50% Agar Agar
Reusable Tattler canning lids are the best option for DIY liquid culture and grain spawn lids. Drill two holes in lid, insert injection port in one and syringe filter in the other. Rubber gasket forms an airtight seal. Purchase with stainless steel canning bands, or use your own.
Syringe filter (13 mm diameter, 0.22 µm). Installed into a canning jar lid, the filter will keep contaminants out, while allowing for adequate gas exchange your liquid cultures.