Liquid culture syringes contain mushroom mycelium suspended in a nutrient broth.  After sterilizing the needle with a flame, transfer your culture through an injection port to sterilized nutrient broth, agar, grains, or bulk substrates. This technique empowers you to maintain relatively sterile technique without expensive lab equipment. Transfer with great success in the open air of your house, but for increased sterility, transfer within a glove box or in front of a flow hood.